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Abstract Detail


Non-Coding RNAs

Barakat, Abdelali [1], Wall, P. Kerr [2], DiLoreto, D. Scott [2], dePamphilis, Claude W. [2], Carlson, John [2].

Analysis of sRNAome in Populus trichocarpa using pyrosequencing.

MicroRNAs (miRNAs) are 20-24 nucleotide small RNAs that negatively control gene expression by cleaving or inhibiting the translation of mRNA of target genes. Among miRNA targets are, genes involved in plant development and stress defense, as well as several genes implicated in different cellular processes. microRNAs have been extensively analyzed in rice and Arabidopsis and partially investigated in other plant species. So far, 109 and 62 miRNA families were identified from Arabidopsis and rice respectively. However, only 33 miRNAs have been identified from Populus; 11 are poplar specific. This number is low compared to the number of miRNA families in Arabidopsis and rice, indicating that many miRNAs still remain to be discovered. The availability of a poplar genome (http://genome.jgi.psf.org/poptrl.home.html) constitutes an opportunity to identify conserved and new poplar specific miRNAs and analyze their diversity in core eudicot and monocot species. We analyzed the sRNAome from leaves and vegetative buds of populus using ultrahigh throughput pyrosequencing. Two libraries corresponding to the two tissues analyzed were constructed and a quarter-plate from each library was sequenced. We will report new Populus-specific microRNAs that have not been identified previosuly, the distribution of their loci in poplar genomes, and putative genes they are targeting.


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1 - Penn State University, Biology, 403 Life sciences building, University Park, PA, 16801, USA
2 - Penn State University, Biology

Keywords:
sRNA
microRNA
Populus

pyrosequencing.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P25003
Abstract ID:2168


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