Growth and Vegetative Development
Ingram, Paul , Malamy, Jocelyn .
Arabidopsis thaliana mutants with altered root system plasticity during mild osmotic stress.
The post-embryonic addition of lateral roots is modulated by changes in several environmental cues, yet little is known about the molecular pathways that mediate these plastic responses. We have focused our attention on the affects of water availability or osmotic stress on root plasticity in the model flowering plant Arabidopsis thaliana. When Arabidopsis accession Columbia (Col-7) seedlings are grown on agar plates containing osmotica, lateral root formation is repressed. This establishes a nice assay to look for mutants that are capable of forming lateral roots under these repressive conditions. Activation tagged lines generated by Detlef Weigel in the Col-7 background are available and represent an excellent resource for screening due to the possibility of isolating both dominant and recessive mutants. Segregating activation tagged lines were screened on plates containing osmotica, and thirteen potential mutants were identified with increased lateral root development. Further characterization of the mutants and progress toward gene identification will be presented.
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1 - University of Chicago, Molecular Genetics and Cell Biology, 1103 E. 57th Street, EBC 209, Chicago, IL, 60637, United States
2 - University of Chicago, Molecular genetics and cell biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM