| Abstract Detail
Reproductive Development Tebbji, Faiza [1], Nantel, André [2], Matton, Daniel P [3]. Transcriptomic profiling of S. chacoense fertilization and embryogenesis. In order to characterize the evolution of the transcriptome during fertilization and early seed development, we constructed cDNA microarrays from 7741 ESTs (82% unigenes) isolated in ovule tissues from the self-incompartible species Solanum chacoense. These arrays were used in a series of experiments with probes derived from ovules isolated from 0 to 22 days after pollination, and from leaves, anthers, and styles of S. chacoense. 1326 genes were differentially expressed (±2 fold change) in ovules after pollination. Analysis of expression ratios between the different tissues enabled the identification of tissue-specific expression patterns. 76.5% of the identified genes were found to be predominantly expressed in ovules. Three distict expression profile patterns could be identified that corresponded to early, middle and late stages of embryo development. The early and middle stages are characterize by proteins involved in cell cycle and DNA processing, and potential regulatory proteins like protein kinases, receptor-like kinases, and transcription factors. Early protein synthesis genes, genes coding for ribosomal proteins and other components of the translation machinery were also highly expressed in embryos during the early stages. Genes for protein degradation were overrepresented later in middle and late stages. As expected, storage protein transcripts accumulated predominantly in the late stage of embryo development. Defense and/or stress response related genes were found in all stages of embryo development. In a second set of experiments, we compared the transcriptome profile following incompatible pollination, MeJA treatment, and wounding of the stigma, since we had previously shown that these treatments could lead to gene activation at a distance in the ovary.
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1 - Université de Montréal, Institut de Recherche en Biologie Végétale (IRBV), 4101 Sherbrooke est, Montréal, Quebec, H1X 2B2, Canada
2 - Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount, Montreal, Quebec, H4P 2R2, Canada
3 - Université de Montréal, Institut de recherche en biologie végétale, 4101 rue Sherbrooke est, Montréal, Québec, H1X 2B2, Canada
Keywords:
Embryo development
Profiling
Signal transduction.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P28005
Abstract ID:210 |
