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Abstract Detail


Membrane Transport

Ayalew, Mentewab [1], Kemp, Megan [1], Mishra, Shreela [1].

Subcellular localization of AtWBC19, an Arabidopsis ABC transporter conferring kanamycin resistance.

The Arabidopsis ABC transporter AtWBC19 was shown to confer resistance to the antibiotic agent, kanamycin. While the precise mechanism involved is still unclear, it is likely to be similar for an entire clade of plant specific ABC transporters. Indeed, AtWBC19 belongs to a group of co-orthologous transporters all of which display highly conserved motifs outside of the ABC domain responsible for ATP binding and hydrolysis. Therefore our aim is to elucidate the subcellular localization of AtWBC19 and to determine the importance of such conserved regions, especially the C-terminal region of the transporter. AtWBC19-GFP fusion constructs as well as single amino-acid mutants of the C-terminal region were generated and transgenic plants recovered. We present preliminary results suggesting that AtWBC19 is a short lived transporter, undergoing rapid endocytosis and degradation in the vacuole.


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1 - Emory University, Biology Department, Rollis Research Center, 1510 Clifton Rd, Rm 2020, Atlanta, GA, 30322, USA

Keywords:
ABC transporter
resistance gene
localization
membrane traffic.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P10012
Abstract ID:2081


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