Systematics Section / ASPT
Won, Hyosig , Lee, Seung-Kyu , Park, Chong-Wook .
A Preliminary Study on the Evolution of Polyploidy in Reynoutria (Polygonaceae) using nuclear xdh gene sequences.
Reynoutria (Polygonaceae) is composed of ca. 3-5 species distributed naturally in China, Korea, Japan, with recent invasion and spreading in Europe and North America. The chromosome numbers of Reynoutria range from 44, 66, 88, and 132 while those of its close relatives are 2n=22, implying polyploidization and genome duplication in the evolution of Reynoutria. Also, extensive hybridization among Reynoutria and even with members of other Polygonaceae genera have been reported. These complex polyploidization and hybridization events cause difficulties in elucidating the evolutionary pathway of the group and delimiting the species boundary. To understand the evolution and speciation of the group, we amplified and sequenced the nuclear xdh gene, which is reported as a single copy nuclear marker. Up to now, we have obtained 18 nuclear haplotypes from the extensive cloning of the PCR-amplified nuclear xdh gene from 9 Reynoutria individuals comprising 3 species, in addition to 2 artificial-hybrid individuals. The number of haplotypes detected per individual ranges from 2 to 6 for the wild ones and 8 for an aritifical hybrid, corresponding with the ploidy level of each individual studied. The 18 haplotypes separate into two distinct lineages in the preliminary phylogenetic analysis, strongly suggesting that the genome of Reynoutria originated from hybridization or genome duplication.
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1 - Seoul National University, Department of Biological Sciences, Seoul, 151-747, Korea
2 - Seoul National University, Department of Biological Sciences, Seoul, 151, Korea
nuclear xdh gene
low copy nulcear markers.
Presentation Type: Oral Paper:Papers for Sections
Location: International Ballroom South/Hilton
Date: Wednesday, July 11th, 2007
Time: 4:15 PM