Perez, Dahlia , Kaplinsky, Nick .
BOBBER1 is a heat shock protein that forms cytoplasmic aggregates in response to heat stress.
BOBBER1 (BOB1) is a novel A. thaliana heat shock protein. BOB1 is required for normal development including patterning during embryogenesis as well as response to heat stress. BOB1 is up-regulated in response to heat shock and bob1 mutants display thermotolerance defects. Previous research has shown that plant small Heat Shock Proteins (sHSP) form heat stress granules and that these granules are critical in plant thermotolerance (Kirschner et al. 2000, Miroshnichenko et al. 2005). sHSPs act as chaperones to help denatured or misfolded proteins reform. In response to heat stress, BOB1 forms aggregates in the cytoplasm of the cells. These aggregates were visualized using BOB1:GFP fusions. The BOB1:GFP fusions consist of a genomic clone of BOB1 that rescues bob1 null mutations. The BOB1:GFP lines were used to define the temperature required for the induction of heat stress granules, the duration of heat shock necessary to induce aggregation, and the longevity of the heat stress granules. Efforts are currently underway to demonstrate the co-localization of BOB1and other sHSPs in the heat stress aggregates.
Kirschner et al, The Plant Journal 24:397-411. (2000)
Miroshnichenko et al, The Plant Journal 41:269-81. (2005)
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1 - Swarthmore College, Biology, 500 College Avenue, Swarthmore, PA, 19081, United States
2 - Swarthmore College, Biology
heat shock protein
heat stress granule.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM