Sparkes, Imogen , Hawes, Chris .
In vivo imaging of Arabidopsis myosins.
The project aim is to identify plant myosins involved in endomembrane trafficking in plants. Far more is known about myosins in mammals and yeast, which are divided into 15 classes with at least 30 members in mammals. Targets for many of these have been identified and movement characteristics and structure have been studied. With the completion of the Arabidopsis genome sequencing project, based on sequence similarity, 17 myosin like genes were identified which fall into 2 classes; 4 members are similar to class VIII myosins and 13 members are similar to class XI myosins.
However, extremely little is known about these Arabidopsis myosins; immunofluorescence studies of MYA2 (class XI) reported to locate to peroxisomes (Hashimoto, K. et al. 2005 Plant Cell Phys. 46:782-789) and functional studies of a T-DNA mutant revealed altered morphology (Holweg, C & Nick, P. 2004 PNAS 101:10488-10493). Our aim is to systematically clone each Arabidopsis myosin and transiently express full length and truncations fused to fluorescent proteins alongside known endomembrane markers in tobacco epidermal cells. This will allow us to address basic questions such as where are they located and do they affect movement of their target?
Studies in yeast have shown that the tail domain is important for interactions with its cargo (Kamal, A. & Goldstein L.S.B. 2002 Curr. Opin. Cell Biol. 14:63-68). Therefore, by making tail fusions we are beginning to ascertain the target of several Arabidopsis myosins.
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1 - Oxford Brookes University, School of Life Sciences, Gipsy Lane, Oxford, Oxfordshire, OX3 0BP, England
Presentation Type: Oral Paper:Papers for Topics
Location: Boulevard B/Hilton
Date: Wednesday, July 11th, 2007
Time: 2:30 PM