| Abstract Detail
Temperature Responses Yu, Chih-Wen [1], Hung, Shu-Hsien [2], Yeh, I-Fang [2], Lin, Chin-Ho [3]. Hydrogen peroxide treatment induces cold tolerance in Arabidopsis via calcium-dependent signaling. Hydrogen peroxide (H2O2) pretreatment effectively alleviated freezing injury in arabidopsis. Pretreatment with 1.0 mM H2O2 lowered the electrolyte leakage of arabidopsis chilled at -3°C for 6 h from 56% to 19% while, the electrolyte leakage of cold-acclimatized (at 4°C for 48 h) seedlings was found to be 16±3%, a trend very similar to that of H2O2 pretreatment (19±3%). Furthermore, after cold treatment, the growths of H2O2-treated and acclimated plants were remarkably less retarded compared with those of the control plants at normal temperature. Hydrogen peroxide induced freezing tolerance of arabidopsis seedlings was lowered by ethyleneglycol-bis(aminoethylether)-N,N´-tetraacetic acid (EGTA) treatment, but not by ruthenium red, indicating that the influx of Ca2+ from extracellular, but not intracellular, pools is an important signal in the induction of tolerance. Pretreatment of Ca2+-channel blocker lanthanum chloride and calmodulin and calcium-dependent protein kinases (CDPKs) antagonist W7 abrogated H2O2-induced freezing tolerance. In contrast, application of voltage-sensitive Ca2+-channel blocker nifedipine and calmodulin antagonist trifluoperazine exhibited no inhibitory effect on the H2O2-induced freezing tolerance. These results suggest that H2O2 pretreatment activates intracellular calcium signaling leading to the development of freezing tolerance in arabidopsis.
Log in to add this item to your schedule
1 - Department of Molecular Biotechnology, Da Yeh University, Chang Hua, Taiwan
2 - Department of Bioindustry Technology, Da Yeh University, Chang Hua, Taiwan.
3 - Department of Life Science, National Chung Hsing University, Taichung, Taiwan
Keywords:
Arabidopsis
Calcium
Cold
Hydrogen peroxide.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P08025
Abstract ID:1957 |
