| Abstract Detail
Cell Walls Schultz, Katelyn [1], Kleinschmidt, Ann [2]. Cloning and expression patterns of Alliaria petiolata class III peroxidases. Class III peroxidases comprise a large multigene family in land plants and the roles of the encoded proteins are diverse. While peroxidase protein sequence information is available, determining the role of specific peroxidase proteins has been challenging. One approach to assist in elucidating function is to identify and sequence peroxidase cDNAs from related plant genera, and determine both the degree of sequence and expression pattern conservation. To this end, we have begun constructing a peroxidase cDNA library from Alliaria petiolata. Using peroxidase specific degenerate primers and 3’Rapid amplification of cDNA ends we cloned and sequenced four class III peroxidase genes from A. petiolata. Three cDNAs were isolated from a stem and one from a leaf specific library. Comparison of these sequences with homologous ones from Arabidopsis thaliana indicated differing degrees of conservation ranging from 95% to 78%. Over a six week period starting in late March, shoot tissue from field grown A. petiolata was isolated and expression patterns of all four peroxidases were determined using RT-PCR. All four peroxidase genes were expressed in the stem tissue, but each had a unique pattern of expression. In addition, after bolting was initiated, differences were observed in the pattern of expression for particular peroxidases within the lower and upper internodes of the stem. Comparison of the stem expression patterns for the A. petiolata peroxidases with those available through the e-FP database for the homologous A. thaliana genes indicated a difference between the homologs both in overall level of and in tissue specificity of expression.
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1 - Allegheny College, Biochemistry
2 - Allegheny College, Biology and Biochemistry, 520 N Main St, Meadville, PA, 16433, USA
Keywords:
peroxidase
alliaria petiolata
expression patterns.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P17038
Abstract ID:1926 |
