Growth and Vegetative Development
Dietrich, Margaret , Swinehart, Ilea .
A Physcomitrella patens mutant defective in cytokinin-mediated vegetative development.
In many mosses, the perception of cytokinin results in a change from filamentous growth to the establishment of a simple ‘meristem’ from which the leafy shoot is derived, thus enabling sexual reproduction and completion of the life cycle. The target of cytokinin action is a single cell and morphological changes are evident in this cell within 3-4 hours after addition of the hormone. The simple ‘meristem’ is fully formed following ~4 days of exposure to cytokinin. Random insertional mutagenesis was used to identify components underlying this cytokinin-induced developmental process in Physcomitrella patens. One of the resultant mutants differentiates more quickly than does the wild-type and produces the target cells for cytokinin action. These cells, however, are largely unable to respond to exogenous cytokinin. TAIL-PCR was used to identify the genomic site disrupted during mutagenesis. Currently homologous recombination is being used to confirm the identity of the disrupted genomic sequence and additional phenotypic and sequence analyses are in progress. Characterization of this mutant defective in the cytokinin response should lead to the identification of an important component of the cytokinin induced-signal transduction pathway underlying the proper development of the moss.
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1 - Grand Valley State University, Biology Dept/Cell & Molecular Biology Program, 1 Campus Drive, Allendale, MI, 49401, USA
2 - Grand Valley State University, Biology Dept
plant growth and development
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM