| Abstract Detail
Mechanisms of Gene Regulation Valliyodan, Babu [1], Libault, Marc [2], Joshi, Trupti [3], Xu, Dong [3], Stacey, Gary [4], Nguyen, Henry [4]. Identification of abiotic stress specific molecular switches for stress tolerance and enhanced seed composition in soybean through high throughput quantitative real time PCR. Several genes are regulated in response to abitoic stresses, such as drought, water logging and salinity, and their gene products function in stress response and tolerance. In the signal transduction network from perception of stress signals to stress-responsive gene expression, various transcription factors (TFs) and cis-acting elements in the stress-responsive promoters function not only as molecular switches for gene expression but also as terminal points of stress signals in the signaling processes. TFs play critical roles in all aspects of a higher plant's life cycle. Even though number of studies has been carried out for functional analysis of individual TFs the function of only a small fraction of these TFs has been revealed so far. Our overall goal is to understand functions of these regulatory switches to dissect their specific role in plant development and stress tolerance. Molecular tailoring of novel TFs has the potential to overcome a number of limitations in creating drought-tolerant transgenic soybean plants with better yield. To understand the expression pattern of different TFs under various stress conditions and seed development, we have developed a unique public resource of transcription factor genes in soybean. We have conducted abiotic stress experiments (drought, water logging and salinity) at vegetative stage V3 and water stress at seed developmental stages with specific focus on seed filling stages. Isolation of total RNA, DNAse treatment, cDNA synthesis and qRT-PCR analysis was conducted with the standard protocols. We discovered several abiotic stress related transcription factors. The stress specificity, expression levels and the probable roles of these unique molecular switches (TFs) will be presented.
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1 - University of Missouri-Columbia, National Center for Soybean Biotechnology and Division of Plant Sciences, 1-31 Ag Building, Columbia, Missouri, 65211, USA
2 - University of Missouri-Columbia, Division of Plant Sciences
3 - University of Missouri-Columbia, Department of Computer Science
4 - University of Missouri-Columbia, National Center for Soybean Biotechnology and Division of Plant Sciences
Keywords:
gene regulation
transcription factor
abiotic stress
Drought
Soybean
seed development.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36044
Abstract ID:1910 |
