| Abstract Detail
Environmental Physiology Purty, Ram [1], Kumar, Gautam [2], Singla-Pareek, Sneh [3], Pareek, Ashwani [4]. Understanding the 'gears of signaling cascade' operative under salinity stress in Brassica contrasting genotypes. Significant inter- and intra-specific variation for salt tolerance exists within the Brassica genera, which has a potential to be exploited for identification of useful candidate genes. Aiming to analyze natural genetic variability in salt tolerance, we have compared the responses of 9 local/exotic genotypes of cultivated Brassica species along with two wild relatives. Comparative physiological and biochemical investigations clearly identified amphidiploids to be relatively more salinity tolerant than diploids. Among the various genotypes analyzed, B. juncea var. CS52 – an amphidiploid, showed minimum affect on shoot and root growth, minimum electrolyte leakage and maximum proline accumulation under salt stress conditions. Transcript profiling for various SOS pathway components in these genotypes indicate towards their strong differential regulation under uninduced as well as salinity-induced conditions. Further, based on limited transcriptome analysis by Northern blotting (employing selected probes for genes having role in stress perception, signaling and response), we observed that the salinity tolerant genotypes maintain relatively higher transcripts of most of these genes under non stress conditions which are otherwise inducible by salinity in other sensitive genotypes such as B. nigra. Generation of comparative heatmap using absolute values of transcript accumulation between the non stressed and salinity stressed seedlings show that the amplitude of both constitutive and salinity induced transcripts is higher in the amphidiploid genotypes which is strongly correlated with their better stress adaptability. These results represent the first investigations aiming at finding out the molecular basis of differential salinity tolerance among Brassica species employing SOS pathway and other stress related genes.
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1 - Jawaharlal Nehru University, School of Life Sciences
2 - Jawaharlal Nehru University, School of Life Sciences, New Delhi, Delhi, 110067, India
3 - International Centre for Genetic Engineering and Biotechnology, Plant Molecular Biology
4 - Jawaharlal Nehru University, School of Life Sciences, Room 401/413, New Delhi, Delhi, 110067, India
Keywords:
salinity
Brassica
transcriptional regulation
contrasting genotypes.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P01003
Abstract ID:189 |
