| Abstract Detail
Chromatin Lee, Hye-Ran [1], Neumann, Pavel [2], Macas, Jiri [3], Jiang, Jiming [2]. Transcription and Evolutionary Dynamics of the Centromeric Satellite Repeats CentO in Rice. The centromere is the chromosomal site for assembly of the kinetochore where spindle fibers attach and sister chromatid cohesion during cell division. The centromeric regions in many complex eukaryotic species contain highly repetitive satellite DNAs. Rice (Oryza sativa) centromeres contain two major classes of repetitive DNA elements: the centromeric CRR retrotransposon and a 155-bp satellite repeat CentO. However, the structural organization and evolution of the centromeric satellite DNA is still poorly understood. We analyzed the structure and organization of the centromeric satellite repeat arrays from rice chromosomes 1 and 8 (Cen1 and Cen8), which contain the largest and smallest CentO arrays, respectively, among the 12 rice chromosomes. We identified higher order structures and local homogenization of the CentO repeats in Cen1 and Cen8. We also developed a chromatin immunoprecipitation-based technique to clone CentO repeats from chromatin containing the centromeric histone H3 variant, CenH3. Sequence variability analysis of the ChIPed CentO repeats revealed a single variable domain within the repeat. We found that both strands of the CentO repeat are transcribed and produce small interfering RNAs, suggesting a potential role in epigenetic chromatin modification. Log in to add this item to your schedule
1 - University of Wisconsin-Madison, Horticulture, 1575 Linden Dr., Madison, WI, 53705, United States 2 - University of Wisconsin-Madison, Horticulture 3 - Institute of Plant Molecular Biology, Czech Republic
Keywords: Transcription Centromere satellite repeat siRNA chromatin immunoprecipitation.
Presentation Type: Plant Biology Abstract Session: P Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton Date: Sunday, July 8th, 2007 Time: 8:00 AM Number: P39005 Abstract ID:1825 |