Systematics Section / ASPT
Morton, Cynthia , Mazie, Abigail .
Angiosperm Phylogeny Based on Nuclear Sequence Information.
XANTHINE dehydrogenase belongs to the molybdenum cofactor dependent hydroxylase class of enzymes. This gene is thought to play an important metabolic role in purine metabolism and has been found to be induced by drought stress. XDH gene sequences for over 200 genera, including several genera of gymnosperms, were analyzed using Bayesian and maximum likelihood methods. Primers were designed for the gene by using animal and plant species. These primers were used to amplify a 1486 bp fragment. Approximately 7 indels were examined ranging from 3-40 bps in length. The number of informative characters per bp is greater than has been reported for rbcL, atpB and matK genes. Traditionally, chloroplast genomic regions have been used for phylogenetic inference in angiosperms. The XDH gene evolves much faster than the traditionally used plastid genes. The nuclear gene tree and the chloroplast gene tree reported are highly congruent and will be discussed. XDH sequences also provide good resolution within angiosperm orders such as the Ericales and Sapindales. A combined analysis of XDH and complementary angiosperm datasets should provide the framework to enhance resolution and internal support in angiosperm phylogenetics.
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1 - Carnegie Museum of Natural History, 4400 Forbes Avenue, Pittsburgh, Pennsylvania, 15213, USA
2 - Carnegie Museum of Natural History, 4400 Forbes Avenue, Pittsburgh, PA, 15213, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Boulevard C/Hilton
Date: Wednesday, July 11th, 2007
Time: 8:45 AM