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Abstract Detail

Systematics Section / ASPT

Drysdale, Sunny S [1], Newman, Shelli [2], Hilu, Khidir W. [3].

Phylogenetic Utility of trnK intron in Caryophyllales.

The matK gene is widely used in plant systematics and has been shown to provide robust phylogenetic trees. This gene is nested within an intron of the gene coding for tRNA-Lysine, trnK. The trnK gene has not been well explored for its potential utility in plant systematics despite its amplification in most matK based studies. We are assessing here the utility of trnK compared to matK in phylogenetic reconstruction of the Caryophyllales. Molecular systematic studies using between 1-4 genes conducted on the order revealed two clades with some families of uncertain affinities. However, support for some of the relationships is low. In this study we used 39 species representing 23 of the 33 caryophyllid families. The alignment of trnK was 1,413 characters and the alignment of matK was 1,680 characters. The combined as well as partitioned matK and trnK data sets were analyzed phylogenetically using maximum parsimony with members of the core eudicots as outgroup taxa. The trnK-based tree was quite comparable to the matK-based tree in topology and bootstrap (BS) support. Both resolved Caryophyllales I and II with BS support of 74% and 86% (trnK), and 76% and 87% (matK) respectively. The only difference between the topologies of the two is a partial resolution of one polytomy in the trnK-based tree. The combined analysis reflected the trnK phylogeny. The robustness of the tree obtained from the trnK data alone was higher than what was obtained with a combined two-gene analysis and is quite similar to a four-gene data analysis. Therefore, it appears that trnK contains useful phylogenetic information in the Caryophyllales.

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1 - Virginia Tech, Biological Sciences, 2119 Derring Hall, Blacksburg, VA, 24061, USA
2 - Virginia Tech, Biological Sciences, Blacksburg, VA, 24061, USA
3 - Virginia Tech, Biological Sciences, Blacksburg, Virginia, 24061, USA


Presentation Type: Poster:Posters for Sections
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P59005
Abstract ID:1755

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