Li, Kunfeng , Jung, Jinwook , Park, Sang Ryoung , Jung, Younsoo , Kim, Minkyun .
Overexpression of the acidic C-terminus region of the HvRAF from barley confers enhanced cadmium tolerance to yeast.
The barley ERF-type transcription factor, HvRAF, has an acidic C-terminus domain containing hemopexin domain signature-like sequence. As an initial study to investigate the function of the C-terminus domain in HvRAF, we cloned the partial HvRAF gene (pHvRAF) corresponding to the C-terminus domain into the yeast/E.coli shuttle vector, pYES2, and then introduced the pYES2::pHvRAF recombinant plasmid into yeast (Saccharomyces cerevisiae strain INVSc1). Yeast transformants harboring pYES2::pHvRAF showed significant tolerance to cadmium stress.
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1 - Seoul National University, School of Agricultural Biotechnology, Sillim-dong san 56-1, Seoul, 151-921, Korea
2 - Seoul National University, School of Agricultural Biotechnology
3 - Seoul National University, School of Agricultural Biotechnology, Seoul, 151-921, Korea
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM