| Abstract Detail
Plant-Pathogen Interactions Jung, Ho Won [1], Tschaplinski, Timothy J [2], Greenberg, Jean T. [3]. Metabolomical Changes during Systemic Acquired Resistance and Some Novel Bioactive Compounds for Defense Responses in Arabidopsis. Plants have evolved complex signal networks for defense responses to counter pathogen attacks. Systemic acquired resistance (SAR), one of well-known responses of plants, is an acquired plant immune response. The response is spread to un-inoculated distal tissues after local pathogen infection or specific activator treatment. It is believed that a mobile signal produced in the local infected tissues can be translocated to distal systemic tissues through the vascular system. Based on this possibility, we expect that a signal molecule(s) can be identified by analyzing the composition of petiole exudates from leaves infected with SAR-inducing pathogen. To pick out an unknown mobile signal molecule(s) for SAR, we collected petiole exudates from leaves of mock-treated and SAR-inducing pathogen-infected wild type Col plants. We applied these bacteria-free exudates into naive Col to verify the biological activity of petiole exudates for inducing defense responses. Petiole exudates from leaves infected plants were active, but exudates from mock-treated plants were not. Our finding suggests that petiole exudates from infected plants may have a higher level of a signal molecule(s) than mock-treated plants. The petiole exudates were subjected to gas chromatography-mass spectrometry (GC-MS) to compare metabolites between mock-treated and pathogen-infected plants. The comparative metabolomic analysis indicates that the metabolome of petiole exudates from leave is extensively reconfigured by SAR-inducing pathogen infection. In addition, we identified several compounds that may play key roles in defense responses in Arabidopsis. In the future, we will characterize the functions of these compounds in plant defense responses. This work was funded by a grant from The National Science Foundation.
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1 - The University of Chicago, Molecular Genetics and Cellular Biology, 1103 East 57th Street, EBC 409, Chicago, IL, 60637, USA
2 - Oakridge National Laboratory
3 - The University of Chicago, Molecular Genetics and Cellular Biology
Keywords:
Metabolomical changes and Bioactive compounds.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15052
Abstract ID:1629 |
