Zhou, Suping .
Identification of salt affected genes in tomato seedlings using a cDNA microarray profiling.
Large-scale gene expression affected by salt stress was analyzed in tomato seedlings (Lycoperson esculentum Mill cv. Money Maker) by a cDNA microarrays (Tom1). The significantly differentially expressed genes (p<0.05) consisted of 1551 and 1757 sequences in root and leaves (cotyledons+ shoot tip), respectively. When further restricted to 2 fold changes, the genes down-regulated in leaves were annotated into five enzyme proteins, those up-regulated were annotated into 17 enzymes. The genes down-regulated in root were annotated into 14 enzymes, and those up-regulated root were annotated into 20 enzymes. There were 3 enzymes that were down-regulated in both leaf and root, and 16 enzymes were up-regulated in both root and leaf. Sixty-six function annotation that contained both up and down-regulated members in both leaf and root. When categorized into different cellular processes, the down-regulated genes were related with cell production, protein translation, electron transport, disease resistance, and homeostasis of oxidative status. Genes associated with ethylene synthesis and down-stream responsive genes, programmed cell death, acid phosphatase (AP), and secondary metabolism were up-regulated. Multiple genes belonging to transcriptional factor, protein kinase, protein phosphatase, proteases, chaperones, membrane transport, and calmodulin, were identified to be differently regulated by salt stress.
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1 - Tennessee State University, Institute of Agricultural and Environmental Research, 3500 John A Merritt Blvd, Nashville, TN, 37209, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM