Seok, Hye-Yeon , Park, Hee-Yeon , Woo, Dong-Hyouk , Lee, Choon-Hwan , Moon, Yong-Hwan .
Transgenic Plants Overexpressing Arabidopsis Zeaxanthin Epoxidase Promote Osmotic-Stress Tolerance.
Zeaxanthin epoxidase (ZEP) is an important enzyme involved in abscisic acid (ABA) biosynthesis as well as in xanthophyll cycle. ABA plays very important role in responses to environmental stress such as drought and high salt, inducing many osmotic stress-responsive genes. In this study, to elucidate the function of ZEP in stress-response, we have generated transgenic plants overexpressing Arabidopsis ZEP gene, and investigated responses of the transgenic plants to salt- and drought-stresses. The transgenic plants were more tolerant to Na+ and Li+ than wild-type plants although responses to the Na+ and Li+ were different. The transgenic plants also showed tolerance to mannitol, suggesting that the transgenic plants are tolerant to drought, and indeed the transgenic plants were tolerant to drought on soil. The transgenic plants had higher expression levels of stress-response genes such as RD29A and Rab18 that are induced by ABA, and higher ABA concentration than wild type. These results suggest that the enhanced stress-tolerance of the transgenic plants may be due to increased ABA by increased ZEP activity. Interestingly, the transgenic plants showed defects in seed development with decreased seed yield and abnormal seed development. To minimize the defect of seed development in the transgenic plants, we are generating another transgenic plants overexpressing ZEP under stress-inducible promoter. Taken together, our results suggest that the ZEP gene would be very useful to generate transgenic plants tolerant to salt- and drought-stresses.
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1 - Pusan National University, Department of Biological Sciences
2 - Pusan National University, Department of Biological Sciences, Busan, 609-735, Korea
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM