Kamigaki, Akane , Hayashi, Makoto , Mikio, Nishimura .
Analysis of Arabidopsis thaliana that was suppressed PEX10 gene expression.
More than 30 peroxisome biogenesis factors (peroxin) have been identified in several organisms. It has been suggested that peroxin functions for various phases of peroxisome biogenesis including peroxisome assembly, matrix protein import, and peroxisome proliferation. The Arabidopsis genome is predicted to encode about 20 homologous proteins to the yeast or mammal peroxins, but their functions remain unclear. So far, we generated transgenic plants that were suppressed each peroxin gene expression using RNAi method and analyzed whether these homologues actually are responsible for peroxisome biogenesis or not. Arabidopsis PEX10 is one of the peroxin that is involved in peroxisomal matrix protein import. Interestingly, phenotype of PEX10 suppressed plant is different from that of wild type plant whereas other peroxins suppressed plants has same phenotype of wild type plant. The leaves of PEX10 suppressed plant were partially pale green after two weeks of germination. The buds showed abnormal morphology and could not open in the time when a plant should bloom. The pistil and stamens were bent in the bud and the siliques were shorter than wild type plant. Then, we analyzed the phenotype of PEX10 suppressed plant in details and discuss novel peroxisomal functions.
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1 - National Institute for Basic Biology, Department of Cell Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM