Systematics Section / ASPT
Gernandt, David S. , Hernandez Leon, Sergio , Vázquez-Vera, Federico .
Phylogenetic inference in Pinus subsection Ponderosae based on cpDNA sequences.
Pinus subsection Ponderosae comprises approximately 17 tree species distributed from western Canada to Nicaragua. Species relationships and delimitations in the group are incompletely understood. An alignment of publicly-available genomic sequences for Pinus thunbergii and P. koraiensis was used to identify highly variable regions and to design primer pairs. We sequenced twenty regions for five species of subsection Ponderosae, and selected six for their relative ability to discriminate species. These six regions had substitution rates two to six times higher than matK. A phylogeny for the subsection was inferred using 7.4 kbp of cpDNA (rbcL, matK, and over 4 kbp of noncoding DNA). Three lineages were recovered: 1) a Sabinianae clade (P. sabiniana, coulteri, and torreyana) in a sister position to P. jeffreyi, 2) a western North American grade (P. ponderosa, arizonica, scopularum, cooperi, durangensis, engelmannii, devoniana, and douglasiana), and 3) a Mesoamerican clade (P. hartwegii, pseudostrobus, montezumae, and maximinoi). Other than the recovery of the Sabinianae clade, other groups did not coincide closely with traditional classifications. Possible pitfalls in using cpDNA to infer species relationships in the group will be discussed.
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1 - Universidad Nacional Autonoma de Mexico, Departamento de Botanica, Instituto de Biologia, ap 70-233, Mexico, DF, 04510, Mexico
2 - Universidad Autonoma del Estado de Hidalgo, Centro de Investigaciones Biologicas, AP 1-69, Plaza Juarez, Pachuca, Hidalgo, 42001, Mexico
Presentation Type: Poster:Posters for Sections
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM