Chen, Yu-Chan , Yang, Fang-Yu , Wang, Yung-Chung , Yen, Hungchen Emilie .
Characterization of salt-induced genes mcCPN and mcSNF in halophyte Mesembryanthemum crystallinum L.
Mesembryanthemum crystallinum L. (ice plant), a halophyte, has been used as a model to study its strategy towards salt stress. Previously, a salt-induced gene mcSKD1 (suppressor of K+ transport growth defect) has been shown to involve in K+ uptake and Na+ compartmentalization. To further characterize the function of mcSKD1, mcCPN (copine) and mcSNF1 (sucrose non-fermented protein kinaseĀ 1) were identified through yeast two-hybrid screen. The main focus of this research is to analyze their functions in salt-tolerant mechanisms of this halophyte. For mcCPN, domain search reveals that it consists of two functional domains, one is copine A domain, similar to A domain of integrins, which is known to mediate protein-protein interactions and the other is RING finger domain possessing E3 ubiquitin ligase activity. Domain search of mcCPN also reveals that it has no C2 domain which is commonly found in mammalian copine family. Therefore, mcCPN can be classified as a plant-specific copine-like protein. The N-terminal sequence of mcCPN has a typical myristoylation site suggesting it is associated with plasma membrane. For mcSNF1, domain analysis reveals that there are three conserved domains including Ser/Thr protein kinase catalytic domain, ubiquitin-associated domain and kinase-associated domain. Moreover, database search shows that mcSNF has high similarity to other Ser/Thr protein kinases. The mRNA expression and protein accumulation of mcCPN and mcSNF1 increase through long-term salt treatments. The protein interactions between mcSKD1, mcCPN, and mcSNF1 under salt stress will be presented.
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1 - National Chung-Hsing University, Taiwan, Department of Life Sciences, No.250, Guoguang Rd., South District, Taichung City 402, Taiwan (R.O.C.)
2 - National Chung-Hsing University, Taiwan, Department of Life Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM