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Abstract Detail


Secondary Metabolism

Cho, Hwa-Young [1], Son, Seok Young [2], Rhee, Hong Soon [2], Yoon, Sung-Yong H. [3], Lee-Parsons, Carolyn W.T. [4], Park, Jeong Jin [2], Park, Jong moon [1].

Synergistic Effects of Sequential treatment with Methyl Jasmonate, Salicylic Acid and Yeast Extract on Benzophenanthridine Alkaloid Accumulation and Protein Expression in Eschscholtzia californica suspension culture.

To develop an optimal bioprocess for secondary metabolite production and explain the bioprocess at the molecular level, we examine the synergistic effects of sequential treatment with methyl jasmonate (MJ), salicylic acid (SA) and yeast extract (YE) on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures. Serial treatment of MJ, SA and YE at 24 h intervals enhanced the accumulation of dihydrosanguinarine (2.5 times) and sanguinarine (5.5 times). This sequential treatment using different signal elicitors was more effective than single elicitor or simultaneous treatment of the elicitors; it induced benzophenanthridine alkaloid accumulation to 917.7 mg/L. Also, (S)-methylcoclaurine-3′-hydroxylase (CYP80B1) and 3′-hydroxy-(S)-N-methylcoclaurine-4′-O-methyltransferase (4′OMT) expressions among enzymes in sanguinarine biosynthetic pathway explained the synergistic effects by sequential treatment of the elicitors. The sequential treatment strategy using elicitors related to different signal transduction pathways can be used to design better processes to increase accumulation of secondary metabolites in plant cell culture. Analysis of protein expression provides the detailed information about metabolite accumulation through the correlated results.


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1 - POSTECH, School of Environmental Science and Engineering, 416 SEE Hyojadong, Pohang, 790-784, KOREA
2 - POSTECH, Chemical Engineering, San 31, Hyoja-dong, Nam-gu, Kyungbuk, Pohang, 790-784, Korea
3 - Exelixis Plant Sciences, Portland, OR, 97224, USA
4 - Northeastern University, Chemical Engineering Department, Boston, MA, 02115, USA

Keywords:
benzophenanthridine alkaloids
methyl jasmonate
salicylic acid
yeast extract.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20006
Abstract ID:154


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