| Abstract Detail
Mineral Nutrition Tehseen, Muhammad [1], Sherson, Sarah [2], Cobbett, Christopher Stewart [2]. Identification and characterization of genes involved in Zn homeostasis. Zinc (Zn) is an essential element in numerous biochemical and physiological processes in plants, yet at elevated concentrations Zn can be toxic. Plants have a number of membrane Zn transporters of the ZIP, CDF and P-type ATPase classes. The P-1B ATPases, AtHMA2 and AtHMA4, in Arabidopsis are essential for Zn homeostasis: an hma2; hma4 double mutant is severely Zn-deficient and can be rescued by the application of exogenous Zn (Hussain et al., 2004). This study aims to identify additional components of Zn homeostasis by suppressing hma Zn-deficient phenotype using a T-DNA activation tag (Weigel et al., 2000). Among 10,000 hma2; hma4 individuals transformed with the activtion tag tested to date 3 showed suppression of Zn-deficient phenotype. These lines are the subject of further genetic, physiological and molecular analysis.
Each of HMA2 and HMA4 have an N-terminal metal binding domain. We also aim to identify novel proteins that interact with these domains by using a yeast-2-hybrid expression system. This study will identify novel functions in Zn homeostasis and improve our current understanding about Zn transport in plants.
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1 - University of Melbourne, Genetics, Parkville, Melbourne, Victoria, 3010, Australia
2 - University of Melbourne, Genetics
Keywords:
Zinc
AtHMA2 & AtHMA4
Activation Tagging.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P11001
Abstract ID:149 |
