Abstract Detail
Environmental Physiology Wu, Hui-Chen [1], Jinn, Tsung-Luo [2]. A Versatile Calmodulin is Temperature-dependent Sensor. The calcium/calmodulin (CaM) transduction pathways participated in early heat shock (HS) response and thermotolerance in rice (Oryza sativa L.) seedlings was investigated. HS initiates Ca2+ signal which is spatially distinct and the heat-stress-increased Ca2+ transients mainly from extracellular source are correlated with CaMs and OsHSP genes expression is confirmed. A significant increase of [Ca2+] i was observed during early HS response at 37C using Fluo-3/acetoxymethyl esters and laser scanning confocal microscopy. Meanwhile exogenous application of Ca2+and A23187 also accelerate OsCaM1 and OsHSP genes expression. For turn on OsHSP genes in response to HS is depended on Ca2+ oscillations, up-down, up-down, which may interpret by OsCaM1 is proposed. OsCaM1-GFP mobilizes from extracellular to cytosol within 10 to 16 min with temperature-dependent manner. When over expression of OsCaM1 in Arabidopsis accelerate HSP genes expression during early HS response. These results provide direct evidence that Ca2+/calmodulin mediated HS signaling is critical for thermotolerant acquisition in planta. Log in to add this item to your schedule
1 - National Taiwan University, Department of Life Science and Institute of Plant Biology, R913 Life Sci Bldg, NTU, Taipei, 10617, Taiwan 2 - National Taiwan University, Department of Life Science and Institute of Plant Biology
Keywords: Heat Shock Calmodulin Ca2+ Oscilllation.
Presentation Type: Plant Biology Abstract Session: P Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton Date: Sunday, July 8th, 2007 Time: 8:00 AM Number: P01030 Abstract ID:1486 |