| Abstract Detail
Secondary Metabolism Lozovaya, Vera [1], Zernova, Olga [2], Lygin, Anatoliy [2], Ulanov, Alexander [2], Li, Susan [3], Hill, Curtis [2], Hartman, Glen [4], Clough, Steve [4], Nelson, Randal [4], Widholm, Jack [2]. Phenolic gene stacking in soybean to enhance disease resistance and health-promoting value. Data will be presented on the metabolic engineering of the soybean phenypropanoid pathway by which isoflavones (beneficial for human and animal health), phytoalexins, plant antimicrobial compounds, and cell wall bound phenolics (that are synthesized and regulated in response to pathogens) targeted at developing soybean lines with resistance to multiple pathogens and enhanced health-improving value.
Glyceollin is a pterocarpan phytoalexin produced de novo in various tissues of soybean plants at the infection sites after pathogenic infection. We have shown that the ability of soybean roots to rapidly produce sufficient amounts of glyceollin in response to the soil-borne fungal pathogen Fusarium solani f. sp. glycines (FSG, the causal agent of soybean sudden death syndrome) is important in providing partial resistance to this fungus. We also generated transformed hairy root lines (harboring foreign phenolic biosynthetic genes) with dramatically decreased isoflavone levels that were not able to produce glyceollin after treatment with FSG, and found that FSG grew more aggressively on these hairy roots than on controls indicating an importance of the phytoalexin, glyceollin, production for root resistance to fungus.
Isoflavones are known to have significant beneficial multifactorial effects on human health and different types of isoflavones can have different health promoting effects which may depend on their concentration. We have recently generated a large number of transgenic soybean plants expressing the single phenolic gene or gene combinations under the soybean seed-specific lectin promoter. Interestingly, several of the transformed lines produced using a mixture of cut out cassettes have shown segregation including producing sublines with the isoflavone transgenes but without hpt (hygromycin resistance) gene used for the selection of transformed cultures.
We are presently expressing multiple genes under different promoters to improve defense responses and nutritional value of soybean and performing metabolomic studies to determine the impact of the genetic modifications on other metabolites.
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1 - UIUC, Crop Sciences, 1201 West Gregory Dr., 284 ERML, Urbana, IL, 61801, USA
2 - UIUC, Crop Sciences
3 - USDA
4 - UIUC, USDA, Crop Sciences
Keywords:
Plant transformation
Soybean
multiple transgene
phenylpropanoids.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20005
Abstract ID:146 |
