| Abstract Detail
Education & Outreach Phillips, Allison [1], Robertson, Amber L. [2], Batzli, Janet [3], Harris, Michelle [3], Miller, Sarah [4]. PCR and gel electrophoresis, moving beyond the technique: Effects of aligning learning goals with assessments. Polymerase chain reaction (PCR) and gel electrophoresis have become common techniques used in undergraduate molecular and cell biology labs. While students enjoy learning these techniques, they often cannot fully comprehend and analyze the outcomes of their experiments due to a disconnect between examples and theory taught in lecture and experiments done in the lab. We have developed and effectively implemented several novel inquiry-based exercises, which integrate the biological concepts or theory of DNA replication with the practical techniques of PCR and gel electrophoresis. Student learning goals and expected outcomes were defined based on broad concepts taught throughout the cell biology lab course and goals specific to the PCR and gel electrophoresis lab. Inquiry based exercises developed to target theory behind the steps of PCR, primer design, gel analysis, and troubleshooting were incorporated into an existing lab unit based on the detection of genetically modified organisms. Evaluative assessments for each exercise were aligned with the expected outcomes and used to measure student learning achievements. The exercises were effective in enhancing student understanding of these concepts as shown by learning gains across all expected student outcomes. In addition, students were receptive to these new materials which helped to uncover and correct prevalent misconceptions.
Log in to add this item to your schedule
1 - University of Wisconsin - Madison, Department of Genetics, 425G Henry Mall, Rm 3340, Madison, WI, 53706, USA
2 - University of Wisconsin - Madison, Department of Horticulture
3 - University of Wisconsin - Madison, Biocore Cirruculum
4 - University of Wisconsin - Madison, Wisconsin Program for Scientific Teaching
Keywords:
undergraduate lab
PCR
gel electrophoresis
GMOs.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P46004
Abstract ID:140 |
