| Abstract Detail
Cell Walls Pauly, Markus [1], Neumetzler, Lutz [2], Kraemer, Florian [3], Obel, Nicolai [2]. Identification and characterization of novel Arabidopsis hemicellulose mutants. The load bearing hemicellulose in the primary cell wall of dicots is xyloglucan (XyG, 25% of the biomass). According to the most accepted wall models XyG interlace cellulose microfibrils and keep them in spatial arrangement by tightening the network via hydrogen bonds as well as preventing cellulose microfibrils to adhere. Structural features of the side-chains and their distribution along the polymer influence physical properties of the wall, alter corresponding biological functions during development, and are responsible for solubility of the polymer particularly digestability by enzymes. XyG is known to be targeted by cell wall loosening enzymes which are involved in cell wall remodelling. Variations in distribution of particular xyloglucan oligosaccharide (XyGO) and their ratios may play a role in biological processes such as cell expansion. We used a semi-automated forward genetic approach to identify Arabidopsis thaliana mutants with altered XyG structures facilitating the unique combination of the specificity of a xyloglucanase and the sensitivity of mass spectrometry. So far, 48 mutants have been identified containing XyG with different degrees of substitution including patterns of O-acetylation as well as novel hitherto unknown structures. The walls of these mutants were further characterized by cell wall monosaccharide analysis and immuno-labeling of various cell wall epitopes. The effect of these structural sidechain alterations on plant morphology and development will be presented.
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1 - DOE- Plant research Lab, Michigan State University, Plant Biology Building, East Lansing, Michigan, 48864, USA
2 - Max-Planck Institute for molecular Plant Physiology, Golm, Germany
3 - DOE-Plant Research Lab, Michigan State University
Keywords:
cell wall
polysaccharide
hemicellulose
xyloglucan
Arabidopsis thaliana
mass spectrometry
glycosylhydrolase.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P17029
Abstract ID:1349 |
