Bae, Chang-Hyu , Abe, Tomoko .
Alteration of amounts of plastid genes in a tobacco (Nicotiana tobacco L. cv. BY-4) albino mutant.
Most of albino mutants have reduced amounts of ptDNA and extensive deletions in their plastid genomes, however, a few mutants have an increase of plastid genes. An albino mutant designated ali was isolated from an M2 population derived from a tobacco (Nicotianan tobacco L. cv. BY-4) plant which had been irradiated with a 14N-ion beam at an early stage of embryogenic development (Bae et al. 2001). Interestingly, it is certain that the albino mutant showed increased amounts of plastid genes. The amounts of chloroplast genes, rbcL, psbA, 23S rDNA and 16S rDNA whose transcription level was significantly reduced were increased two- or three-fold in the mutant, although the amounts of nuclear gene (25S rDNA) were similar in both ali and wild-type plants. Although cell plan area of albino plant was smaller than those of wild type, no significant difference in the numbers of chloroplasts per mesophyll cell was detected. Polyploidy level was similar in both ali and wild-type plants. The results suggest that ali is a new albino mutant in which amounts of chloroplast gene is affected.
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1 - Sunchon National University (SCNU), Division of Plant Production Sciences, 315 Maegok-dong, Suncheon, Jeonnam, 540-742, South Korea
2 - RIKEN (The Institute pf Physical and Chemical Research), Plant Functions Lab.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM