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Abstract Detail


Phycological Section

Ruck, Elizabeth [1], Andersen, Robert [2], Theriot, Edward [3], Jansen, Robert K. [4], Cattalico, Rose Ann [5], Rocap, Gabrielle [6], Julius, Mathew [7], Draisma, Stefano [8], Kawai, Hiroshi [9], Ashworth, Matt P. [10], Zhengqiu, Cai [4], Ong, Han [5].

Current Progress Towards an Algal Heterokont Tree of Life.

Currently, 14 classes of heterokont algae are recognized, and they include over 100,000 described species (as many as one million total species has been estimated by experts). Common members include brown seaweeds and the diatoms. Remarkably, despite two centuries of light microscopic study, 50 years of electron microscopic study, and 20 years of molecular investigations, phylogenetic relationships among the 14 classes remains unknown. This project is estimating relationships among the heterokont classes by generating two large molecular data sets. First, DNA sequences of seven nuclear, mitochondrial, and chloroplast genes from 270 heterokont algal species and 30 non-photosynthetic relatives are being gathered. Second, entire plastid genomes from 30 species will be sequenced. During the first nine months of this project we have devoted most of our time to growing cultures and performing a pilot study of 30 taxa. This pilot study is being performed to make a final selection of the seven genes (3 nuclear, 2 mitochondrial, and 2 plastid genes) for expanded taxon sampling. Our preliminary studies are initially exploring a larger number of potential genes, including the nuclear genes SSU rRNA, actin, heat shock protein 70 and 90, alpha and beta tubulin, the plastid genes rbcL, psbC, cfxQ, and the mitochondrial genes nad2, 4, and 5. In addition, we have developed a fosmid cloning approach for plastid genome sequencing. This approach has enabled the sequencing of plastid genomes within several lineages of algal heterokonts that have not yet been analyzed.


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1 - University of Texas at Austin, Section of Integrative Biology, Austin, TX, 78712, USA
2 - Bigelow Laboratory for Ocean Sciences, P.O. Box 475, 180 McKown Point Road, West Boothbay Harbor, Maine, 04575-0475, USA
3 - University of Texas at Austin, Texas Memorial Museum, 2400 Trinity St., Austin, Texas, 78705, USA
4 - University of Texas Austin, Section of Integrative Biology, 1 University Station, A6700, Austin, Texas, 78712-7640, USA
5 - University of Washington, Department of Biology, Box 355325, Seattle, WA, 98195, USA
6 - University of Washington, School of Oceanography, Box 357940, Seattle, WA, 98195, USA
7 - St. Cloud State University, Department of Biological Sciences, 720 Fourth Avenue South, 262 Robert H. Wick Science Bldg., ?St. Cloud, MN, 56301, USA
8 - Nationaal Herbarium Nederland, Leiden University branch (NHN-L), P.O. Box 9514, Leiden, The Netherlands
9 - Kobe University Research Center for Inland Seas (KURCIS)?Kobe Universi, 1-1 Rokkodai, Nadaku, Kobe, 657-8501, Japan
10 - University of Texas at Austin, Section of Integrative Biology, 1 University Station, A670, Austin, Texas, 78712-7640, USA

Keywords:
Algal Heterokonts
Plastid
Genomes
phylogeny.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P55002
Abstract ID:1340


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