Kanno, Satomi , Rai, Hiroki , Hayashi, Yoshitake , Tanoi, Keitaro , Nakanishi, Tomoko .
Real-time imaging of inorganic phosphate movement in a soybean plant through a new system developed.
Plants have several strategies to adapt to phosphate supply from environment. In recent years, numerous genes induced by phosphate starvation have been reported to study the strategies more in detail. However, molecular mechanism related to movement or chemical phosphate form in plants is very limited. Therefore, first of all, to know the chemical form of phosphate during the uptake in a plant, we measured total phosphate and inorganic phosphate concentration in each tissue by ICP and colorimetric method, respectively, under phosphate starvation and re-supplied condition. From these measurements, organic as well as inorganic phosphate concentrations ware obtained. Then, we applied 32P labeled isotope to the plant and measured the amount of phosphate moving within a plant from radioactivity. Comparing the results obtained from 32P activity with those by chemical analyses, it was found that the chemical form of phosphate translocates from root to leaves was an inorganic one. With this result, we studied the real-time movement of inorganic phosphate in a plant through a new 32P imaging system we developed. Under starvation, different accumulation manner of phosphate was found, for example, higher accumulation was observed in an old trifoliates compared with that of control. The decrease of uptake speed from root to up-ground part under phosphate starvation was found within a few hours, suggesting the decrease of transcription level of the phosphate transporters. However, accumulation speed at leaf tissue was not changed in a few hours. In this study, we could show that our new imaging system was able to analyze how inorganic phosphate accumulates in soybean tissues quantitatively in the process of recovery of phosphate starvation.
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1 - The University of Tokyo, Graduate school of Agricultural and Life Sciences Department of Applied Biological Chemistry, Lab. of Radioisotope Plant Physiology, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
2 - The University of Tokyo, Graduate school of Agricultural and Life Sciences
CCD imaging system.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM