Unable to connect to database - 10:56:52 Unable to connect to database - 10:56:52 SQL Statement is null or not a SELECT - 10:56:52 SQL Statement is null or not a DELETE - 10:56:52 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 10:56:52 Unable to connect to database - 10:56:52 SQL Statement is null or not a SELECT - 10:56:52

Abstract Detail


Protein Modification and Turnover

Gilkerson, Jonathan [1], Brown, Jessica [2], Callis, Judy [3].

Characterization of a Novel, Recessive Allele of CUL1 that Disrupts SCF Regulation at the C-terminus of CUL1.

Auxin regulates many aspects of plant growth and development and has been shown to signal via selected proteolysis of Aux/IAA proteins. Auxin facilitates the interaction between the Aux/IAAs and an SCF E3 ubiquitin ligase. The SCF ubiquitin ligase is a multi-subunit protein complex consisting of four subunits: SKP1 (ASK1 in Arabidopsis), a Cullin family member such as CUL1, a RING finger protein RBX1, and a targeting recognizing F-box protein. Here, we report the characterization of a novel, recessive allele of CUl1, cul1-7, isolated from a genetic screen in Arabidopsis thaliana (Col) designed to identify plants defective in degradation of a fusion protein with an Aux/IAA degron, IAA1:LUC. One mutation was identified that exhibited very slow IAA1:LUC degradation as compared to the 10-12min half-life of IAA1:LUC in the progenitor line (Callis line 362) as measured in cyclohexamide chases. Genetic mapping placed the mutation within an 0.8 cM genetic interval that included the CULLIN1 gene. We sequenced the coding region, 5’ UTR, and 3’ UTR of CUl1 and identified a mutation. Allelism experiments with axr6-3 confirmed that this locus was responsible for the mutant phenotype. Mutant alleles of CUl1 have been isolated previously (Development 127:23-32; Plant J 43:371-83; Plant Phys 143:684-96), but the mutations are all at the N-terminus and affect binding to the SCF subunit SKP1. No alleles that affect subunit interactions at the CUL1 C-terminus have been described to date. We believe the location of this mutation within CUL1 and its recessive nature will allow this allele to be used as a tool to dissect regulation of SCF activity at the C-terminus of CUL1.


Log in to add this item to your schedule

1 - University of California-Davis, Plant Biology Graduate Group - Section of Molecular and Cellular Biology, One Shields Avenue, Davis, CA, 95616
2 - University of California-Davis, Section of Molecular and Cellular Biology
3 - University of California-Davis, Plant Biology Graduate Group - Section of Molecular and Cellular Biology

Keywords:
auxin
SCF
Aux/IAA.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P37012
Abstract ID:1246


Copyright © 2000-2007, Botanical Society of America. All rights