| Abstract Detail
Plant-Pathogen Interactions Perry, Greg [1], Reinprecht, Yarmilla [2], Chan, John [2], Pauls, K. Peter [2]. Towards the Identification of Common Bacterial Blight Resistance Genes in Phaseolus vulgaris. Common bacterial blight (CBB) is a significant disease problem in dry bean (Phaseolus vulgaris) crops throughout the world that results in losses in yield and seed quality. This disease presents itself as brown lesion of seeds, leaves and pods and is caused by Xanthomonas axonopodis pv. phaseoli. Recently, a CBB-resistant cultivar, OAC-Rex was developed from a cross between P. vulgaris and a CBB-resistant accession of Phaseolus acutifolius. Another CBB-resistant line, HR67, was produced from a separate cross between P. vulgaris and P. acutifolius. OAC-Rex represents the first CBB resistant cultivar released in North America, however the genes responsible for this resistance not yet been identified.
To begin the search for genes associated with CBB resistance in these cultivars, binary-bacterial artificial chromosome (BiBAC) libraries were created by ligating high molecular weight DNA from OAC-Rex and HR67 into the BiBAC2 vector. The libraries were screened with CBB resistance-associated molecular markers identified by previous studies, and the identified clones were analyzed using a gel-based restriction fingerprinting method for assembly into contigs. The fragments at the extreme ends of the contigs will be used to re-probe the libraries and expand the coverage of the contig. The unique clones will be transiently expressed in susceptible bean lines using Agrobacterium tumefaciens, and the plants will be infected with X. axonopodis. Clones containing genes for CBB resistance should cause a significantly reduced X. axonopodis-induced lesion. The inserts will be sequenced and analyzed to identify potential resistance genes.
Log in to add this item to your schedule
1 - University of Guelph, Department of Plant Agriculture, Guelph, ON, N1G2W1, Canada
2 - University of Guelph, Department of Plant Agriculture
Keywords:
Bacterial Blight
Xanthomonas axonopodis
Phaseolus vulgaris
BiBAC
Resistance.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15048
Abstract ID:1242 |
