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Abstract Detail


Mineral Nutrition

Rai, Hiroki [1], Kanno, Satomi [2], Hayashi, Yoshitake [3], Ohya, Tomoyuki [2], Tanoi, Keitaro [2], Nakanishi, Tomoko [2].

Real-time autoradiography of labeled compounds in a living plant.

Real-time imaging of compounds in a living plant is expected to provide the physiological activity of a plant more in detail, such as transport of compounds or signals. The radioisotope imaging in a plant, autoradiography, is widely performed using an X-ray film or an IP (imaging plate), which is basically a destructive method because a plant has to be harvested and fixed for exposure, therefore, further experiment using the same plant cannot be carried out. To perform nondestructive real-time imaging of a plant, we developed an imaging system, which enabled to image conventional radioisotopes in a plant, such as 32P or 14C. With this system, beta-rays emitted from the radioisotopes were converted to visible light by a scintillator and was introduced to a photon counting camera. The resolution was about 100 μm in 5 cm×5 cm view area and the sensitivity was 10 times higher than that by an IP, under the same exposure time. We present real-time images of the inorganic phosphate and calcium (32P and 45Ca) moving in the ground part from the root. Based on this system, we are challenging to improve the resolution as high as to image the labeled compounds in the cell level. With the study of scintillator thickness deposited on a fiber base plate and an application of tapered fiber plate for enlargement of the image, we installed these devices to a fluorescent microscope, which enabled to obtain both radioisotope and the fluorescent or brightfield images in sliced tissues, simultaneously. The observation area was 2 mm×2 mm and the resolution was about 25 μm. Now we are trying to combine this imaging system with microdissection devices for cutting out and gene expression analysis of the target organization, identified from the concentration map of the labeled compounds.


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Related Links:
Lab. of Radioisotope Plant Physiology The University of Tokyo


1 - The University of Tokyo, Lab. of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
2 - The University of Tokyo, Lab. of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences
3 - The University of Tokyo, Lab. of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences 

Keywords:
real-time autoradiography
CCD imaging system
radioisotope
autoradiography.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P11013
Abstract ID:1213


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