Kolar, Elizabeth A. , Burnett, Emily N. , Miller, Katherine R. , Hunter, Richard , Hunter, Kimberly .
Quantification of Nordihydroguaiaretic Acid (NDGA) in the Three Ploidy Levels of Larrea tridentata.
Larrea is one of the dominant perennials in the deserts of North and South America. North American Larrea tridentata has three ploidy levels in three distinct regions: Chihuahuan Desert –diploid; Sonoran Desert – tetraploid; Mojave Desert – hexaploid. Nordihydroguaiaretic acid (NDGA) is one of the most widely investigated phytochemicals within this genus. NDGA is a tetrahydroxy lignan found in the leaves at a concentration of 5-10% dry weight. NDGA is a powerful antioxidant that inhibits cancer, microbes, fungi, and viruses. Two methods were investigated to determine NDGA concentration of the three ploidy levels of Larrea using small samples (<1 g). The first method was a classic organic derivatization that utilized gas chromatography (GC), and the alternative method was a methanol extraction of NDGA that was quantified using high performance liquid chromatography (HPLC). The GC method produced inconsistent results. The HPLC method was able to quantify NDGA concentration in plants using only 20 mg (two leaves). Fresh and dried samples of the three different ploidy levels were analyzed and results suggest NDGA concentrations are variable across the landscape.
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1 - Salisbury University, Department of Biological Sciences, 1101 Camden Avenue, Salisbury, Maryland, 21801, USA
2 - Salisbury University, Department of Chemistry, 1101 Camden Avenue, Salisbury, Maryland, 21801, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Williford C/Hilton
Date: Wednesday, July 11th, 2007
Time: 4:00 PM