Mechanisms of Gene Regulation
Hill, Kristine , Wang, Huai , Perry, Sharyn E .
A Transcriptional Repression Motif in the MADS-factor AGL15 is Involved in Recruitment of Histone Deactylase Complex Components.
AGAMOUS-Like 15 (AGL15) encodes a MADS-box transcription factor that is preferentially expressed in the plant embryo, and is believed to be an important regulator in embryonic developmental programs. Research in our lab has identified a number of downstream targets of AGL15, and while some of these target genes are induced in response to AGL15, others are repressed. A number of direct target genes have been analyzed that exhibit strong responses to AGL15 levels in vivo, yet in vitro, AGL15 binds only weakly. Taken together these data suggested that AGL15 may form hetero-dimers, or ternary complexes with other proteins, thus modulating AGL15ís specificity and function in planta. The Yeast Two-Hybrid system has been used to address this question and putative interactors of AGL15 have been identified. This presentation will focus on AGL15ís interaction with a member of the SIN3 histone deactylase complex (HDAC), SIN3 Associated Protein of 18 KD (SAP18), and the potential biological consequences of AGL15ís association with this complex in planta. A motif within the C-terminal domain of AGL15, which is conserved among putative orthologs of AGL15, appears to function as a repression domain in vivo. What is more, preliminary data suggests that the aforementioned motif might mediate AGL15ís association with SAP18, thus providing a possible mechanism for AGL15ís role in regulating gene expression.
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1 - University of Kentucky, Plant & Soil Sciences, 1405 Veterans Drive, Plant Sciences Building Rm 352, Lexington, KY, 40546, USA
2 - Univeristy Wisconsin, Department of Genetics
3 - University of Kentucky, Department of Plant & Soil Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM