| Abstract Detail
Plant-Pathogen Interactions Peethambaran, Bela [1], Wang, Lin [2], Mitra, Raka [2], Botanga, Chris [2], Qi, Yiping [2], Glazebrook, Jane [2]. Arabidopsis 14-3-3 (nu) is involved in defense against Pseudomonas syringae. Plants have evolved complex and sophisticated mechanisms to survive attack by microbial pathogens. In response to pathogen exposure, multiple host signaling networks are activated resulting in the differential expression of hundreds of plant genes. In order to identify host genes that are required for pathogen defense, we have used a reverse-genetics approach involving the inactivation of pathogen-induced plant genes. Using microarray experiments, we identified a set of Arabidopsis thaliana genes that are transcriptionally induced in response to Pseudomonas syringae (Psm4326). We determined whether inactivation of each gene by a T-DNA insertion resulted in enhanced disease susceptibility to P. syringae. From this screening, we identified a 14-3-3 (nu) protein that is required for defense against P. syringae. We are currently conducting functional analyses on this protein to better understand the role of 14-3-3 proteins in disease signaling.
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1 - University of Minnesota, Plant Biology, Cargill building, Room 350, 1500 Gortner Avenue, St. Paul, MN, 55108, USA
2 - University of Minnesota, Plant Biology
Keywords:
Arabidopsis thaliana
14-3-3 protein
P. syringae
pathogen defense.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15047
Abstract ID:1117 |
