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Abstract Detail

Emerging Technologies

Arnold, Nicole [1], Bauer, Teresa [1], Collingwood, Trevor [2], DeKelver, Russell [2], Doyon, Yannick [2], Gao, Zhifang [1], McCaskill, Dave [1], Miller, Jeffrey [2], Mitchell, Jon [1], Moehle, Erica [2], Rebar, Ed [2], Rock, Jeremy [2], Rowland, Lynn [1], Shukla, Vipula [1], Simpson, Matt [1], Skokut, Michiyo [1], Urnov, Fyodor [2], Worden, Sarah [1], Yau, Kerrm [1], Zhang, Lei [2].

Application of Designed Zinc-Finger Protein Technology in Plants.

The primary sequence of the genome at endogenous loci can be altered with high efficiency in mammalian cells using designed zinc finger nucleases (ZFNs; Urnov et al. Nature 435: 646). Ongoing studies indicate that a precisely-placed double-strand break (DSB) induced by engineered ZFNs can stimulate integration of long DNA stretches into a predetermined genomic location in human cells, resulting in site-specific gene addition. Zinc finger protein technology represents a significant breakthrough relative to the ability to edit and engineer genomes in a precise manner. In this presentation, results from a collaboration between Dow AgroSciences LLC and Sangamo Biosciences that is focused on applications of designed zinc-finger protein technology in plant species will be described. Multiple zinc-finger proteins, including zinc-finger nucleases and zinc-finger transcription factors, have been designed to target specific genes in model and agriculturally important plant species. Validation of this technology and examples of its utility for plant biotechnology will be discussed.

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1 - Dow AgroSciences LLC, Discovery R&D, 9330 Zionsville Road, Building 306, Indianapolis, INDIANA, 46268, USA
2 - Sangamo Biosciences, Inc.

Zinc finger nuclease
Targeted integration
homologous recombination

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44015
Abstract ID:1114

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