| Abstract Detail
Hormone Biology Kim, Eun Hye [1], Kim, Youn Shic [1], Koo, Yeon Jong [2], Park, Su-Hyun [1], Nahm, Baek Hie [1], Song, Sang Ik [1], Choi, Yang Do [2], Cheong, Jong-Joo [2], Chung, Yong-Yoon [3], Kim, Ju-Kon [1]. Transgenic Overexpression of Arabidopsis JASMONIC ACID CARBOXYL METHYL TRANSFERASE Alters Spikelet Development of Rice. Jasmonic acid (JA), salicylic acid (SA), and ethylene signaling pathways are integrated in plant development and defense response. The Arabidopsis Jasmonic acid carboxyl methyltransferase gene (AtJMT) was linked to the Ubi1 promoter and transformed into rice. Levels of MeJA were increased by 3 to 10-fold in leaves and flowers of transgenic plants as compared to those of nontransgenic plants. Ubi1:AtJMT plants grow and develop normally except that flower organ numbers are severely altered. Carpel, anther, lodicules, lemma and palea numbers significantly increased or decreased as compared to nontransgenic controls. MeJA-treated nontransgenic plants showed a similar floral organ number alterations suggesting that increased levels of MeJA caused changes in phenotype of the Ubi1:AtJMT plants. By using the 300K Rice Tilling Microarray and RT-PCR, we found that more than 30 genes including leucine rich repeat proteins, photosynthesis related proteins and transcription factors with AP2, WRKY and HEX domains, are up- or down-regulated in flowers of Ubi1:AtJMT plants.
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1 - Myongji University, School of Biotechnology and Environmental Engineering
2 - Seoul National University, School of Agricultural Biotechnology
3 - Korea University, School of Life Science and Biotechnology
Keywords:
Transgenic rice
methyljasmonic acid
jasmonic acid
spikelet development.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P35034
Abstract ID:1103 |
