| Abstract Detail
Mechanisms of Gene Regulation Azhakanandam, Kasi [1], Qu, Rongda [1]. Isolation and characterization of exon junction complex (EJC) in plants and study the function of two EJC core proteins Mago and Y14. The recently discovered exon-exon junction complex (EJC), which deposits upstream of exon-exon junctions after intron splicing has been found to be most responsible for enhanced gene expression by the introns in mammals. The EJC is deposited 20-24 nucleotides upstream of exon-exon junctions in a non-sequence specific manner and contains at least 11 protein components with a molecular weight around 335 kDa. Intron-mediated enhancement (IME) of gene expression is also remarkable in plants, and highly conserved homologs of some EJC proteins were found in plants. However, there is little study on potential plant EJC and its role in gene expression and development in plants. Consequently, we are curious to investigate two aspects: 1) Does EJC complex exist in plants? 2) What are the key roles of the core proteins of EJC, such as Mago and Y14 in plants?
A y14::TAP and TAP::y14 fusion constructs were made and inserted in GVG expression system and stable transgenic Arabidopsis lines were generated to isolate the EJC complex. These constructs are also being used for transient expression of the proteins in Arabidopsis and TEV-B transgenic tobacco plants. Transgenic Arabidopsis lines, contained mago or y14 RNAi construct driven by CaMV 35S or TA29 tapetum specific promoter, were generated to study the function of the two core proteins of the EJC in plant. Results obtained from molecular, biochemical and mass spectroscopy studies will be presented at the meeting.
Log in to add this item to your schedule
1 - North Carolina State University, Department of Crop Science, Raleigh, NC 27695
Keywords:
EJC
Mago
Y14.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36025
Abstract ID:1031 |
