| Abstract Detail
Cell Walls Zhu, Jinming [2], Alvarez, Sophie [1], Marsh, Ellen [1], LeNoble, Mary [2], Chen, Sixue [1], Nguyen, Henry [2], Wu, Yajun [3], Schachtman, Daniel P. [1], Sharp, Robert [2]. Cell wall proteome in the maize primary root growth zone: region-specific responses to water deficit. Kinematic studies have revealed different responses of cell elongation to water deficit in distinct regions of the maize primary growth zone, with elongation being maintained preferentially towards the apex. Previous work showed that longitudinal cell wall extensibility is enhanced in the apical region of the growth zone in water-stressed compared to well-watered roots, thereby facilitating the maintenance of cell elongation despite reduced turgor pressure. The biochemical basis of this response is poorly understood, and we are using a proteomics approach to gain a comprehensive understanding of how cell wall protein (CWP) composition changes in association with the differential growth responses to water deficit in the different regions. To date we have examined water soluble and loosely ionically-bound (fraction A) and tightly ionically-bound (fraction B) CWP, and the results reveal major and predominantly region-specific changes in both profiles between well-watered and water-stressed roots. For fractions A and B, respectively, a total of 110 and 70 water-deficit responsive proteins were identified. The fraction B protein population was distinct from fraction A, with only two of the identified proteins in common. Functional roles of the regulated proteins and integration of the results with microarray analysis of CWP gene expression will be discussed. (Supported by National Science Foundation grant no. DBI-0211842.)
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1 - Donald Danforth Plant Science Center
2 - University of Missouri-Columbia, Division of Plant Sciences
3 - Utah State University, Plants, Soils and Biometeorology
Keywords:
cell wall
maize
proteome
roots
water deficit.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P17017
Abstract ID:1021 |
