| Abstract Detail
Genetics Section Zhuang, Yan [1], Adams, Keith [2]. Allelic Variation of Gene Expression in Populus F1 Hybrids. Plant hybridization can induce phenotypic novelty and speciation as well as gene expression changes. Populus hybrids provide a good system to study interspecific hybridization and its genetic and molecular consequences. In this project, we determined the allelic variation of gene expression in Populus trichocarpa × Populus deltoides F1 hybrids using a single-base primer extension assay. Among 28 genes analyzed so far, 11 showed strong bias at the expression level for one of the two alleles, and the expression patterns differed between leaves and stems for five genes. These results suggest differential regulation of the two parental alleles in the Populus hybrids. To determine if the allelic expression biases were caused by hybridization we compared the ratios of species-specific transcripts between hybrids and the parental species. Cis-regulation was inferred for some genes. This research has illuminated aspects of regulation of gene expression upon interspecific hybridization.
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1 - University of British Columbia, Botany Department, 6270 University Blvd, Botany Department, Vancouver, BC, V6T1Z4, Canada
2 - University of British Columbia, Botanical Garden, Centre for Plant Research, and Botany Department, 6270 University Blvd, Vancouver, BC, V6T1Z4, Canada
Keywords:
hybridization
gene expression
allelic variation
Populus.
Presentation Type: Oral Paper:Papers for Sections
Session: CP14
Location: Lake Huron/Hilton
Date: Monday, July 9th, 2007
Time: 3:15 PM
Number: CP14009
Abstract ID:1000 |
