| Abstract Detail
Emerging Technologies Torney, Francois [1], Trewyn, Brian [2], Lin, Victor [2], Wang, Kan [3]. Nanoparticles mediated plant genetic transformation. Plant genetic engineering relies mostly on biolistic and Agrobacterium-mediated transformation technologies. Both techniques allow DNA delivery into plant cells and subsequent integration into the genome. Recently, the development of nanomaterials such as mesoporous silicate nanoparticles (MSN) was shown to deliver marker genes into animal cells (Radu et al., J. Am. Chem. Soc. 126, 13216-13217, 2004). The distinct feature of this nanoparticle is that it can both deliver DNA as well as chemicals encapsulated in the particles. Controlled release of the filling substance is also possible using this material (Gruenhagen et al., Appl. Spectrosc. 59, 424-431, 2005). Here we show that this material can be used for transforming tobacco mesophyll protoplasts, intact leaves and immature maize embryos. Transgene expression was observed both transiently and stably. We can induce gene expression in transgenic plants using MSN to deliver a transgene-inducing chemical under controlled-release conditions. In addition, we can deliver two different biogenic species into plant cells and release the encapsulated chemical in a controlled manner to trigger the expression of co-delivered transgene in the cell. We envision that further development of this system offers new possibilities to plant biotechnology.
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Related Links:
ISU Center for Plant Transformation
Dr. Lin's group
1 - Iowa State University, Agronomy
2 - Iowa State University, Chemistry
3 - Iowa State University, Agronomy, G405 Agronomy Hall, Ames, Iowa, 50011-1010, USA
Keywords:
Plant transformation.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44013
Abstract ID:811 |
