| Abstract Detail
Growth and Vegetative Development Sbabou, Laila [1], Podkowinski, Jan [1], hulshout, jeltie [1], Bucciarelli, Bruna [2], Miller, Sue [2], Allan, Deborah [1], Vance, Caroll [2]. Scarecrow (SCR) Mediates Root Development in Lupinus albus and Medicago truncatula. EST sequencing of white lupine (Lupinus albus) phosphorus stressed cluster roots revealed the expression of a scarecrow like gene (LaSCR), belonging to the Gras family of transcription factors. Transcript abundance as measured by RT-PCR and RNA blots was low in all tissues assayed. SCR is known to regulate the asymmetric cell division required for the formation of ground tissues (cortex/endodermis) during root development in many species. In order to determine the biological function of SCR in L.albus and Medicago truncatula we developed a RNAi construct for delivery to roots via Agrobacterium rhizogenes transformation. The RNAi construct was made using a 450 bp segment of the LaSCR coding region. Medicago truncatula root development was impaired upon suppression of LaSCR expression. Cluster root development in white lupine transformed with LaSCR RNAi is currently being evaluated.
This research was supported by USDA/NRI Grant Nr. 2005-35100-16007 and USDA/ARS
 L. Sbabou was supported by Fulbright program
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1 - University of Minnesota, Soil Water and Climate, Saint Paul, MN, 55108, USA
2 - University of Minnesota, United States Department of Agriculture, Agricultural Research Service, and Department of Agronomy and Plant Genetics
Keywords:
Lupinus albus
Medicago truncatula
Scarecrow TF
RNAi
root development.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26023
Abstract ID:498 |
